SubtiBank SubtiBank
yrpC [2018-02-12T22:12:12.000Z]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

yrpC [2018-02-12T22:12:12.000Z]

glutamate racemase
locus
BSU26810
pI
6.74
mw
29.73 kDa
protein length
265 aa Sequence Blast
gene length
795 bp Sequence Blast
function
peptidoglycan precursor biosynthesis
product
glutamate racemase
essential
no
ec
5.1.1.3
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]

    • Gene

    Coordinates
    2,738,308 → 2,739,105

    The protein

    Catalyzed reaction/ biological activity

  • L-glutamate = D-glutamate (according to Swiss-Prot)

    • Protein family

  • aspartate/glutamate racemases family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|36FF9F097682D8BA8D7359BFEAF71ACE8E9E90D8|RacE]

    • Structure

  • [PDB|2JFN] (from ''Escherichia coli'', 36% identity, 54% similarity) [Pubmed|17568739]

    • Expression and Regulation

    Operons

    genes
    [gene|BBEE8CEC868D3B781BEA50C45935BC810EA7A4FB|yrpC]
    description
    [Pubmed|22383849]

    regulation

  • expressed only in minimal medium, not in complex medium [Pubmed|15347750]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A867 (yrpC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/867 NBRP B. subtilis, Japan]
  • BKE26810 (Δ[gene|BBEE8CEC868D3B781BEA50C45935BC810EA7A4FB|yrpC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE26810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACTTCTCACGCTTTCT, downstream forward: _UP4_TGAATGTAAATGAAAAAGAC
  • BKK26810 (Δ[gene|BBEE8CEC868D3B781BEA50C45935BC810EA7A4FB|yrpC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK26810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACTTCTCACGCTTTCT, downstream forward: _UP4_TGAATGTAAATGAAAAAGAC

    • References

  • 15347750,22383849,25777674